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FEBS Lett. 2006 Jul 24;580(17):4121-5. Epub 2006 Jun 30.

Transient suppression of PPARgamma directed ES cells into an osteoblastic lineage.

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  • 1Research Center for Animal Life Science, Shiga University of Medical Science, Tsukinowa-cho, Seta, Ohtsu 520-2192, Japan.


Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the addition of any osteogenic factors. We found that PPARgamma-siRNA downregulated the expression of aP2 mRNA and lipid accumulation, whereas it upregulated the expression of osteocalcin and calcium deposition. These results suggested that ES cells were directed into an osteoblastic lineage. Therefore, transient suppression using PPARgamma-siRNA may be a novel tool to induce differentiation of ES cells into osteoblasts.

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