Abstract
Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the addition of any osteogenic factors. We found that PPARgamma-siRNA downregulated the expression of aP2 mRNA and lipid accumulation, whereas it upregulated the expression of osteocalcin and calcium deposition. These results suggested that ES cells were directed into an osteoblastic lineage. Therefore, transient suppression using PPARgamma-siRNA may be a novel tool to induce differentiation of ES cells into osteoblasts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / cytology
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Adipocytes / physiology
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Animals
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Calcification, Physiologic / drug effects
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Calcification, Physiologic / physiology
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Calcium / metabolism
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cell Line
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Cell Lineage / drug effects
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Cell Lineage / physiology*
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Embryo, Mammalian / cytology
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Embryo, Mammalian / physiology*
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Mice
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Osteoblasts / cytology
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Osteoblasts / physiology*
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Osteopontin
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PPAR gamma / antagonists & inhibitors
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PPAR gamma / genetics
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PPAR gamma / metabolism*
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RNA, Messenger / biosynthesis
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RNA, Small Interfering / genetics
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RNA, Small Interfering / pharmacology
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Sialoglycoproteins / biosynthesis
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Stem Cells / cytology
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Stem Cells / physiology*
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Transcription Factors / biosynthesis
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Up-Regulation / drug effects
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Up-Regulation / physiology
Substances
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PPAR gamma
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RNA, Messenger
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RNA, Small Interfering
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Sialoglycoproteins
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Spp1 protein, mouse
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Transcription Factors
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Osteopontin
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Calcium