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J Struct Biol. 2007 Jan;157(1):117-25. Epub 2006 Jun 2.

FREALIGN: high-resolution refinement of single particle structures.

Author information

  • Howard Hughes Medical Institute, Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA. niko@brandeis.edu

Abstract

The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author's web page.

PMID:
16828314
[PubMed - indexed for MEDLINE]
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