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J Virol Methods. 1991 Jun;33(1-2):39-46.

Inactivation of HIV-1 by trypsin and its use in demonstrating specific virus infection of cells.

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  • 1Cancer Research Institute, University of California, School of Medicine, San Francisco 94143-0128.

Abstract

HIV-1 was found sensitive to inactivation by low concentrations of trypsin. The use of trypsin was valuable for assessing non-specific binding of HIV virions to CD4+ cells. This effect was also helpful for eliminating input virus in experiments studying HIV infection of cells in vitro.

PMID:
1682337
[PubMed - indexed for MEDLINE]
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