Mol Cell. 2006 Jul 7;23(1):71-81.

Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model.

Jasiak AJ, Armache KJ, Martens B, Jansen RP, Cramer P.

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Department of Chemistry and Biochemistry, Gene Center, Ludwig-Maximilians-University of Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.

Abstract

We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face includes a HRDC domain in subunit C17 that is translated by 35 A and rotated by 150 degrees compared to its Pol II counterpart. The HRDC domain is essential in vivo, folds independently in vitro, and, unlike other HRDC domains, shows no indication of nucleic acid binding. Thus, the HRDC domain is a functional module that could account for the role of C17 in Pol III promoter-specific initiation. During elongation, C17/25 may bind Pol III transcripts emerging from the adjacent exit pore, because the subcomplex binds to tRNA in vitro.

PMID:: 16818233; [PubMed - indexed for MEDLINE]

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Cited by 20 PubMed Central articles

Recessive mutations in POLR3B, encoding the second largest subunit of Pol III, cause a rare hypomyelinating leukodystrophy. [Am J Hum Genet. 2011]
Recessive mutations in POLR3B, encoding the second largest subunit of Pol III, cause a rare hypomyelinating leukodystrophy.
Tétreault M, Choquet K, Orcesi S, Tonduti D, Balottin U, Teichmann M, Fribourg S, Schiffmann R, Brais B, Vanderver A, et al. Am J Hum Genet. 2011 Nov 11; 89(5):652-5. Epub 2011 Oct 27.
Mutations in POLR3A and POLR3B encoding RNA Polymerase III subunits cause an autosomal-recessive hypomyelinating leukoencephalopathy. [Am J Hum Genet. 2011]
Mutations in POLR3A and POLR3B encoding RNA Polymerase III subunits cause an autosomal-recessive hypomyelinating leukoencephalopathy.
Saitsu H, Osaka H, Sasaki M, Takanashi J, Hamada K, Yamashita A, Shibayama H, Shiina M, Kondo Y, Nishiyama K, et al. Am J Hum Genet. 2011 Nov 11; 89(5):644-51. Epub 2011 Oct 27.
Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth. [Genes Dev. 2011]
Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth.
Blattner C, Jennebach S, Herzog F, Mayer A, Cheung AC, Witte G, Lorenzen K, Hopfner KP, Heck AJ, Aebersold R, et al. Genes Dev. 2011 Oct 1; 25(19):2093-105. Epub 2011 Sep 22.

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Structures reported by this article

X-Ray Structure Of Rna Polymerase Iii Subcomplex C17-C25

PDB: 2CKZ

Source: Saccharomyces cerevisiae

Method: X-Ray Diffraction

Resolution: 3.2 Å

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