Display Settings:

Format

Send to:

Choose Destination
Genetics. 2006 Sep;174(1):511-8. Epub 2006 Jul 2.

Introns regulate RNA and protein abundance in yeast.

Author information

  • 1Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA. kjuneau@stanford.edu

Abstract

The purpose of introns in the architecturally simple genome of Saccharomyces cerevisiae is not well understood. To assay the functional relevance of introns, a series of computational analyses and several detailed deletion studies were completed on the intronic genes of S. cerevisiae. Mining existing data from genomewide studies on yeast revealed that intron-containing genes produce more RNA and more protein and are more likely to be haplo-insufficient than nonintronic genes. These observations for all intronic genes held true for distinct subsets of genes including ribosomal, nonribosomal, duplicated, and nonduplicated. Corroborating the result of computational analyses, deletion of introns from three essential genes decreased cellular RNA levels and caused measurable growth defects. These data provide evidence that introns improve transcriptional and translational yield and are required for competitive growth of yeast.

PMID:
16816425
[PubMed - indexed for MEDLINE]
PMCID:
PMC1569799
Free PMC Article

Images from this publication.See all images (2)Free text

F igure  1.—
F igure  2.—
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk