Novel object recognition in Apoe(-/-) mice improved by neonatal implantation of wild-type multipotential stromal cells

Exp Neurol. 2006 Sep;201(1):266-9. doi: 10.1016/j.expneurol.2006.03.023. Epub 2006 Jun 30.

Abstract

Multipotential bone marrow stromal cells (MSCs) from wild-type (Wt) or apolipoprotein E deficient (Apoe(-/-)) mice were implanted into the cerebral ventricles of Apoe(-/-) mice. MSCs from Wt mice continued expressing apoE up to 6 months after implantation and were associated with enhanced novel object recognition and increased microtubule-associated protein 2 (MAP2) immunoreactivity in the dentate gyrus. These data show that MSCs can be used to distinguish developmental from post-developmental effects of a gene knockout and support their therapeutic potential for neurodegenerative diseases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Apolipoproteins E / genetics
  • Apolipoproteins E / metabolism*
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • Bone Marrow Transplantation / methods
  • Cerebral Ventricles / metabolism
  • Cerebral Ventricles / surgery
  • Dentate Gyrus / metabolism
  • Female
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microtubule-Associated Proteins / metabolism
  • Stromal Cells / cytology
  • Stromal Cells / metabolism
  • Stromal Cells / transplantation*
  • Totipotent Stem Cells / cytology
  • Totipotent Stem Cells / metabolism
  • Totipotent Stem Cells / transplantation*

Substances

  • Apolipoproteins E
  • Microtubule-Associated Proteins
  • Mtap2 protein, mouse