Aim: To investigate the effect of chemokine-like factor superfamily 8 (CKLFSF8) on proliferation and epidermal growth factor receptor (EGFR) expression of tumor cells.
Methods: Expression of CKLFSF8 mRNA in BGC823 and K562 cells was assayed by RT-PCR. Then target gene was transfected into tumor cells by lipid method, and cell growth was observed by inverted microscope. Cell proliferation was determined by MTT assay and expression of EGFR on tumor cells was determined by immunocytochemical technique.
Results: CKLFSF8 was expressed in the two tumor cells. After transfection, cell proliferation was inhibited (P<0.05) and the expression of EGFR of tumor cells was also discovered to be inhibited (P<0.05).
Conclusion: The finding shows that cell proliferation and expression of EGFR of tumor cells can be inhibited by transfection of CKLFSF8. The new chemokine may has a bright prospect in the treatment of tumor.