Abstract
Recombinant Streptococcus mutans glucan-binding protein D (rGbpD) was incorporated into poly(lactic-co-glycolic acid) (PLGA) microspheres which then were surface-coated with chitosan. The microspheres, with a mean diameter of ca. 1.8 microm, were intranasally administered in rats. There were elevated salivary immunoglobulin A and serum immunoglobulin G antibody responses to rGbpD, as well as lower molar caries scores in immunized animals as compared to sham immunized ones. The chitosan-coated PLGA microspheres are thus potentially useful for antigen delivery in dental caries vaccination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Intranasal
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Animals
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Carrier Proteins / chemistry*
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Chitosan / chemistry*
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Enzyme-Linked Immunosorbent Assay
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Kinetics
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Lactic Acid / chemistry*
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Lectins / chemistry*
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Male
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Microspheres*
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Polyglycolic Acid / chemistry*
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polymers / chemistry*
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Rats
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Rats, Sprague-Dawley
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Saliva / metabolism
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Streptococcus mutans / immunology*
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Streptococcus mutans / metabolism*
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Surface Properties
Substances
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Carrier Proteins
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Lectins
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Polymers
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glucan-binding proteins
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polyglycolic Acid
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Lactic Acid
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Chitosan