Nat Med. 2006 Jul;12(7):852-5. Epub 2006 Jun 25.

Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing.

Source

Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA.

Erratum in

Nat Med. 2006 Oct;12(10):1220.

Abstract

The sensitivity of conventional DNA sequencing in tumor biopsies is limited by stromal contamination and by genetic heterogeneity within the cancer. Here, we show that microreactor-based pyrosequencing can detect rare cancer-associated sequence variations by independent and parallel sampling of multiple representatives of a given DNA fragment. This technology can thereby facilitate accurate molecular diagnosis of heterogeneous cancer specimens and enable patient selection for targeted cancer therapies.

PMID:: 16799556; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

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MeSH Terms

Substances

DNA, Neoplasm

Grant Support

R01CA098185/CA/NCI NIH HHS/United States

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