Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2006;335:115-33.

Use of self-quenched, fluorogenic LUX primers for gene expression profiling.

Author information

  • 1Research and Development, Invitrogen Corporation, Carlsbad, CA, USA.


Application of a real-time detection system based on a novel primer design in gene expression profiling is described. In this system, called LUX (Light Upon eXtension), the generation of signal is based on a single fluorescent dye molecule that is attached to an oligonucleotide close to the 3'-end. A primer design software is available that identifies LUX primer pairs based on a set of rules for optimum signal development. The use of LUX fluorogenic primers to determine the expression patterns of various transcripts during differentiation in the P-19 mouse neuronal model is described.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk