Curr Opin Biotechnol. 2006 Aug;17(4):353-8. Epub 2006 Jun 15.

Enhancement of soluble protein expression through the use of fusion tags.

Source

Protein Expression Laboratory, Research Technology Program, SAIC-Frederick, Inc, NCI-Frederick, Frederick, MD 21702, USA.

Abstract

The soluble expression of heterologous proteins in Escherichia coli remains a serious bottleneck in protein production. Although alteration of expression conditions can sometimes solve the problem, the best available tools to date have been fusion tags that enhance the solubility of expressed proteins. However, a systematic analysis of the utility of these solubility fusions has been difficult, and it appears that many proteins react differently to the presence of different solubility tags. The advent of high-throughput structural genomics programs and advances in cloning and expression technology afford us a new way to compare the effectiveness of solubility tags. This data should allow us to better predict the effectiveness of tags currently in use, and might also provide the information needed to identify new fusion tags.

PMID:: 16781139; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Recombinant Fusion Proteins

Grant Support

N01-CO-12400/CO/NCI NIH HHS/United States

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. [BMC Biotechnol. 2006]
A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production.
Cabrita LD, Dai W, Bottomley SP. BMC Biotechnol. 2006 Mar 1; 6:12. Epub 2006 Mar 1.
Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO. [Protein Sci. 2006]
Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO.
Marblestone JG, Edavettal SC, Lim Y, Lim P, Zuo X, Butt TR. Protein Sci. 2006 Jan; 15(1):182-9. Epub 2005 Dec 1.
Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli. [Protein Expr Purif. 2005]
Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli.
Tsunoda Y, Sakai N, Kikuchi K, Katoh S, Akagi K, Miura-Ohnuma J, Tashiro Y, Murata K, Shibuya N, Katoh E. Protein Expr Purif. 2005 Aug; 42(2):268-77.
Review SUMO fusion technology for difficult-to-express proteins. [Protein Expr Purif. 2005]
Review SUMO fusion technology for difficult-to-express proteins.
Butt TR, Edavettal SC, Hall JP, Mattern MR. Protein Expr Purif. 2005 Sep; 43(1):1-9. Epub 2005 Apr 9.
Review Making the most of affinity tags. [Trends Biotechnol. 2005]
Review Making the most of affinity tags.
Waugh DS. Trends Biotechnol. 2005 Jun; 23(6):316-20.

See reviews... See all...

Cited by 24 PubMed Central articles

Isotope labeling in insect cells. [Methods Mol Biol. 2012]
Isotope labeling in insect cells.
Saxena K, Dutta A, Klein-Seetharaman J, Schwalbe H. Methods Mol Biol. 2012; 831:37-54.
Expression screening of fusion partners from an E. coli genome for soluble expression of recombinant proteins in a cell-free protein synthesis system. [PLoS One. 2011]
Expression screening of fusion partners from an E. coli genome for soluble expression of recombinant proteins in a cell-free protein synthesis system.
Ahn JH, Keum JW, Kim DM. PLoS One. 2011; 6(11):e26875. Epub 2011 Nov 2.
Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system. [Microb Cell Fact. 2011]
Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system.
Douillard FP, O'Connell-Motherway M, Cambillau C, van Sinderen D. Microb Cell Fact. 2011 Aug 9; 10:66. Epub 2011 Aug 9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Enhancement of soluble protein expression through the use of fusion tags.
Enhancement of soluble protein expression through the use of fusion tags.
Curr Opin Biotechnol. 2006 Aug ;17(4):353-8. Epub 2006 Jun 15 .

PubMed
Antibacterial evaluation of a collection of norfloxacin and ciprofloxacin deriva...
Antibacterial evaluation of a collection of norfloxacin and ciprofloxacin derivatives against multiresistant bacteria.
Int J Antimicrob Agents. 2006 Jul ;28(1):19-24. Epub 2006 Jun 14 .

PubMed
Acupuncture for patients with chronic neck pain.
Acupuncture for patients with chronic neck pain.
Pain. 2006 Nov ;125(1-2):98-106. Epub 2006 Jun 14 .

PubMed
Complete nerve deafness after abuse of co-proxamol.
Complete nerve deafness after abuse of co-proxamol.
Lancet. 1991 Aug 17 ;338(8764):446-7.

PubMed
Health care costs in the last year of life--the Dutch experience.
Health care costs in the last year of life--the Dutch experience.
Soc Sci Med. 2006 Oct ;63(7):1720-31. Epub 2006 Jun 14 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: