Methods Mol Biol. 2006;326:235-45.

The use of combined immunohistochemical labeling and in situ hybridization to colocalize mRNA and protein in tissue sections.

Source

Rheumatology Unit, Repatriation General Hospital, Daw Park, Adelaide, Australia.

Abstract

This chapter explores the combination of a nonradioactive in situ hybridization technique to detect mRNA with an imunnohistochemical labeling technique for use in formalin-fixed, paraffin-embedded, or frozen tissue sections. This technique allows the combination of detecting mRNA by in situ hybridization with immunohistochemical detection of a protein product or a cell surface marker without using any radioactive procedures. This technique is ideal for use on tissue sections when the aim is to identify which cells are producing a secreted protein product, such as a cytokine.

PMID:: 16780206; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Review

MeSH Terms

Substances

LinkOut - more resources

Supplemental Content

Save items

Related citations in PubMed

Combined immunohistochemical labeling and in situ hybridization to colocalize mRNA and protein in tissue sections. [Methods Mol Biol. 2000]
Combined immunohistochemical labeling and in situ hybridization to colocalize mRNA and protein in tissue sections.
Smith MD, Parker A, Wikaningrum R, Coleman M. Methods Mol Biol. 2000; 123:165-75.
A nonradioactive method of in situ hybridization that uses riboprobes and paraffin-embedded tissue and its combination with immunohistochemistry. [Diagn Mol Pathol. 1997]
A nonradioactive method of in situ hybridization that uses riboprobes and paraffin-embedded tissue and its combination with immunohistochemistry.
Smith MD, Triantafillou S, Parker A, Wikaningrum R, Coleman M. Diagn Mol Pathol. 1997 Feb; 6(1):34-41.
In situ hybridization for vasopressin mRNA in the human supraoptic and paraventricular nucleus; quantitative aspects of formalin-fixed paraffin-embedded tissue sections as compared to cryostat sections. [J Neurosci Methods. 1996]
In situ hybridization for vasopressin mRNA in the human supraoptic and paraventricular nucleus; quantitative aspects of formalin-fixed paraffin-embedded tissue sections as compared to cryostat sections.
Lucassen PJ, Goudsmit E, Pool CW, Mengod G, Palacios JM, Raadsheer FC, Guldenaar SE, Swaab DF. J Neurosci Methods. 1996 Jan; 64(1):133.
Review In situ hybridization: detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples. [Expert Rev Mol Diagn. 2004]
Review In situ hybridization: detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples.
Mabruk MJ. Expert Rev Mol Diagn. 2004 Sep; 4(5):653-61.
Review In situ detection of microRNAs in paraffin embedded, formalin fixed tissues and the co-localization of their putative targets. [Methods. 2010]
Review In situ detection of microRNAs in paraffin embedded, formalin fixed tissues and the co-localization of their putative targets.
Nuovo GJ. Methods. 2010 Dec; 52(4):307-15. Epub 2010 Aug 17.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

The use of combined immunohistochemical labeling and in situ hybridization to co...
The use of combined immunohistochemical labeling and in situ hybridization to colocalize mRNA and protein in tissue sections.
Methods Mol Biol. 2006 ;326:235-45.

PubMed
Development and validation of a pediatric Crohn's disease activity index.
Development and validation of a pediatric Crohn's disease activity index.
J Pediatr Gastroenterol Nutr. 1991 May ;12(4):439-47.

PubMed
Indications and outcomes of free tissue transfer to the lower extremity in child...
Indications and outcomes of free tissue transfer to the lower extremity in children: review.
J Reconstr Microsurg. 2006 Apr ;22(3):173-81.

PubMed
Imaging molecular expression on vascular endothelial cells by in vivo immunofluo...
Imaging molecular expression on vascular endothelial cells by in vivo immunofluorescence microscopy.
Mol Imaging. 2006 Jan-Mar ;5(1):31-40.

PubMed
The role of a HSV thymidine kinase stimulating substance, scopadulciol, in impro...
The role of a HSV thymidine kinase stimulating substance, scopadulciol, in improving the efficacy of cancer gene therapy.
J Gene Med. 2006 Aug ;8(8):1056-67.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

The use of combined immunohistochemical labeling and in situ hybridization to colocalize mRNA and protein in tissue sections.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information