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Plant Physiol. 2006 Aug;141(4):1248-54. Epub 2006 Jun 9.

CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis.

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  • 1Lehrstuhl für Genetik, Technische Universität München, D-85350 Freising, Germany.

Erratum in

  • Plant Physiol. 2007 Nov;145(3):1086.

Abstract

Camalexin represents the main phytoalexin in Arabidopsis (Arabidopsis thaliana). The camalexin-deficient phytoalexin deficient 3 (pad3) mutant has been widely used to assess the biological role of camalexin, although the exact substrate of the cytochrome P450 enzyme 71B15 encoded by PAD3 remained elusive. 2-(Indol-3-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid (dihydrocamalexic acid) was identified as likely intermediate in camalexin biosynthesis downstream of indole-3-acetaldoxime, as it accumulated in leaves of silver nitrate-induced pad3 mutant plants and it complemented the camalexin-deficient phenotype of a cyp79b2/cyp79b3 double-knockout mutant. Recombinant CYP71B15 heterologously expressed in yeast catalyzed the conversion of dihydrocamalexic acid to camalexin with preference of the (S)-enantiomer. Arabidopsis microsomes isolated from leaves of CYP71B15-overexpressing and induced wild-type plants were capable of the same reaction but not microsomes from induced leaves of pad3 mutants. In conclusion, CYP71B15 catalyzes the final step in camalexin biosynthesis.

PMID:
16766671
[PubMed - indexed for MEDLINE]
PMCID:
PMC1533948
Free PMC Article

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