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Nat Genet. 2006 Jun;38 Suppl:S2-7.

Approaches to microRNA discovery.

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  • 1Hubrecht Laboratory, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.


MicroRNAs (miRNAs) are noncoding RNAs that can regulate gene expression. Several hundred genes encoding miRNAs have been experimentally identified in animals, and many more are predicted by computational methods. How can new miRNAs be discovered and distinguished from other types of small RNA? Here we summarize current methods for identifying and validating miRNAs and discuss criteria used to define an miRNA.

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