Format

Send to

Choose Destination
See comment in PubMed Commons below
Microbiology. 2006 Jun;152(Pt 6):1601-7.

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture.

Author information

  • 1Department of Microbiology and Immunology, UAMS, 4301 West Markham, Little Rock, AR 72205, USA. oconnellcatherine@uams.edu

Abstract

A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4-30%). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Ingenta plc
    Loading ...
    Write to the Help Desk