Chem Commun (Camb). 2006 Jun 14;(22):2359-61. Epub 2006 May 9.

Phosphoester-transfer mechanism of an RNA-cleaving acidic deoxyribozyme revealed by radioactivity tracking and enzymatic digestion.

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Department of Biochemistry and Biomedical Sciences, Department of Chemistry, McMaster University, 1280 Main St. W. Hamilton, ON L8N 3Z5, Canada.

Abstract

A convenient method involving 32P-labeling of an RNA substrate at the cleavage site and subsequent enzymatic digestion of cleavage products via phosphatases reveals that pH4DZ1--an RNA-cleaving deoxyribozyme with optimal activity at pH 4--forms a 5'-cleavage fragment with 2',3'-cyclic phosphate group and a 3'-cleavage fragment with 5'-OH group.

PMID:: 16733579; [PubMed - indexed for MEDLINE]

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