Abstract
Dopamine potently increased calcium mobilization in Chinese hamster ovary cells expressing human dopamine D2Long receptors (CHO-D2L cells), and increased guanosine-5'-O-(3-[35S]thio)-triphosphate binding to CHO-D2L cell and rat striatal membranes. These effects of dopamine were blocked by the dopamine D2 receptor antagonist (-)raclopride. In contrast to the findings of a recent controversial study, phencyclidine, ketamine and dizocilpine (MK-801) lacked dopamine D2 receptor full agonist, partial agonist and antagonist activity in these assays, suggesting their psychotomimetic effects, and activity in rodent models of schizophrenia, are associated with N-methyl-d-aspartate receptor blockade rather than a direct interaction with dopamine D2 receptors.
MeSH terms
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Animals
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Binding, Competitive / drug effects
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CHO Cells
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Calcium / metabolism
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Cell Membrane / drug effects
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Cell Membrane / metabolism
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Cricetinae
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Cricetulus
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Dizocilpine Maleate / pharmacology*
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Dopamine / pharmacology
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Dopamine Agonists / pharmacology
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Dopamine Antagonists / pharmacology
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Dose-Response Relationship, Drug
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Gene Expression / genetics
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Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
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Humans
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Ketamine / pharmacology*
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Phencyclidine / pharmacology*
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Raclopride / pharmacology
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Rats
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Receptors, Dopamine D2 / genetics
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Receptors, Dopamine D2 / metabolism*
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Sulfur Radioisotopes
Substances
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Dopamine Agonists
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Dopamine Antagonists
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Receptors, Dopamine D2
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Sulfur Radioisotopes
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Guanosine 5'-O-(3-Thiotriphosphate)
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Raclopride
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Ketamine
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Dizocilpine Maleate
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Phencyclidine
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Calcium
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Dopamine