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    Appl Bioinformatics. 2006;5(2):99-109.

    MultiPrimer: software for multiplex primer design.

    Lee C, Wu JS, Shiue YL, Liang HL.

    Source

    Department of Computer Science and Engineering, National Sun Yat-Sen University, Kaohsiung, Taiwan. cnlee@mail.cse.nsysu.edu.tw

    Abstract

    This study presents a novel application, MultiPrimer, to assist users to design multiplex PCR primers. MultiPrimer adopts an algorithm based on a genetic algorithm and can efficiently solve the multi-objective optimisation problem. A match pattern model is proposed to speed up examination of the specificity constraint. MultiPrimer is able to find a set of primer pairs simultaneously for a set of gene family sequences and multiple targets for a single cDNA sequence. A public version of the MultiPrimer software is available from http://edith.cse.nsysu.edu.tw/new/software/MultiPrimer.htm.

    PMID:
    16722774
    [PubMed - indexed for MEDLINE]

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