Format

Send to:

Choose Destination
See comment in PubMed Commons below
Med J Aust. 2006 May 15;184(10):507-10.

B-cell antigen D8/17 is a marker of rheumatic fever susceptibility in Aboriginal Australians and can be tested in remote settings.

Author information

  • 1Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia. zintah@bigpond.com

Abstract

OBJECTIVE:

To test the B-cell antigen D8/17 as a marker of past rheumatic fever (RF) in a predominantly Aboriginal Australian population, and to evaluate technical modifications to allow its use in remote settings.

DESIGN AND SETTING:

Cross-sectional survey in a remote Aboriginal community, a regional tertiary referral hospital and a tertiary paediatric centre in Melbourne.

PARTICIPANTS:

106 people, including three with acute RF, 38 with a history of past RF, 20 relatives of these people, and 45 healthy controls.

MAIN OUTCOME MEASURE:

D8/17 expression in B cells.

RESULTS:

Blood was collected from each participant and the expression of D8/17 and CD19 in each sample was analysed by flow cytometry. The mean proportion of D8/17-positive B cells was 39.3% (SD, 11.8) in patients with previous RF, 22.5% (SD, 5.2) in first-degree relatives, 11.6% (SD, 7.2) in controls, and 83.7% (SD, 10.1) in patients with acute RF (analysis of variance test between means, P = 0.001). A cut-off of 22.1% of D8/17-positive B cells to indicate past RF yielded the highest percentage of correct results (95.4%). Delayed staining of whole blood (mean, 0.55 days; SD, 0.2) gave equivalent results to immediate staining, but the D8/17 assay on peripheral blood mononuclear cells was unreliable.

CONCLUSIONS:

The B-cell antigen D8/17 accurately identifies Australians with a past history of RF, and the assay is feasible in remote settings with access to facilities capable of performing D8/17 staining within half a day of sample collection.

Comment in

PMID:
16719750
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Australasian Medical Publishing Company
    Loading ...
    Write to the Help Desk