Biosci Biotechnol Biochem. 2006 May;70(5):1181-7.

Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of Aspergillus oryzae.

Source

Research Institute, Gekkeikan Sake Co., Ltd, Kyoto, Japan. h_ishida@gekkeikan.co.jp

Abstract

The glucoamylase-encoding gene (glaB) promoter should be very useful for recombinant protein production in solid-state fermentation (SSF) of Aspergillus oryzae. A 97-bp fragment containing the cis-element of the glaB promoter was inserted into the glaA promoter, which was little expressed in SSF. The chimeric promoter showed about a 24-fold increase in promoter activity in SSF. Eight copies of the 97-bp fragment were tandemly fused with the glaB promoter. The improved promoter showed about a 4.6-fold increase in promoter activity in SSF. The glaB gene was overexpressed under control of the improved glaB promoter in SSF. Recombinant glucoamylase production reached about 1524 mg/kg-broth for 2 d. The improved glaB promoter should be very useful for overproduction of a recombinant protein in SSF of A. oryzae.

PMID:: 16717420; [PubMed - indexed for MEDLINE]

Free full text

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

J-STAGE, Japan Science and Technology Information Aggregator, Electronic

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

Identification of functional elements that regulate the glucoamylase-encoding gene (glaB) expressed in solid-state culture of Aspergillus oryzae. [Curr Genet. 2000]
Identification of functional elements that regulate the glucoamylase-encoding gene (glaB) expressed in solid-state culture of Aspergillus oryzae.
Ishida H, Hata Y, Kawato A, Abe Y, Suginami K, Imayasu S. Curr Genet. 2000 Jun; 37(6):373-9.
Establishment of a hyper-protein production system in submerged Aspergillus oryzae culture under tyrosinase-encoding gene (melO) promoter control. [Appl Microbiol Biotechnol. 2001]
Establishment of a hyper-protein production system in submerged Aspergillus oryzae culture under tyrosinase-encoding gene (melO) promoter control.
Ishida H, Matsumura K, Hata Y, Kawato A, Suginami K, Abe Y, Imayasu S, Ichishima E. Appl Microbiol Biotechnol. 2001 Oct; 57(1-2):131-7.
Nucleotide sequence of an alternative glucoamylase-encoding gene (glaB) expressed in solid-state culture of Aspergillus oryzae. [Gene. 1998]
Nucleotide sequence of an alternative glucoamylase-encoding gene (glaB) expressed in solid-state culture of Aspergillus oryzae.
Hata Y, Ishida H, Ichikawa E, Kawato A, Suginami K, Imayasu S. Gene. 1998 Jan 30; 207(2):127-34.
Review Impact of Aspergillus oryzae genomics on industrial production of metabolites. [Mycopathologia. 2006]
Review Impact of Aspergillus oryzae genomics on industrial production of metabolites.
Abe K, Gomi K, Hasegawa F, Machida M. Mycopathologia. 2006 Sep; 162(3):143-53.
Review Biotechnological advantages of laboratory-scale solid-state fermentation with fungi. [Appl Microbiol Biotechnol. 2004]
Review Biotechnological advantages of laboratory-scale solid-state fermentation with fungi.
Hölker U, Höfer M, Lenz J. Appl Microbiol Biotechnol. 2004 Apr; 64(2):175-86. Epub 2004 Feb 13.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of ...
Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of Aspergillus oryzae.
Biosci Biotechnol Biochem. 2006 May ;70(5):1181-7.

PubMed
Posthospital course and outcome in schizophrenia.
Posthospital course and outcome in schizophrenia.
Arch Gen Psychiatry. 1991 Mar ;48(3):247-53.

PubMed
Survey on the clinical skills of osteopathic medical students.
Survey on the clinical skills of osteopathic medical students.
J Am Osteopath Assoc. 2006 May ;106(5):296-301.

PubMed
Video assessment of supraglottic airway orientation through the perilaryngeal ai...
Video assessment of supraglottic airway orientation through the perilaryngeal airway in pediatric patients.
Anesth Analg. 2006 Jun ;102(6):1685-8.

PubMed
Diabetes susceptibility.
Diabetes susceptibility.
CMAJ. 2006 May 23 ;174(11):1597-8; author reply 1598.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: