Saliva of patients with Type 1 diabetes: effect of smoking on activity of lysosomal exoglycosidases

Oral Dis. 2006 May;12(3):278-82. doi: 10.1111/j.1601-0825.2005.01190.x.

Abstract

Objective: The aim of our study was to evaluate the influence of smoking on the activity of N-acetyl-beta-hexosaminidase (HEX), its isoenzymes A (HEX-A) and B (HEX-B) and beta-galactosidase (GAL), in the saliva of patients with Type 1 diabetes.

Methods: In the supernatant HEX and its isoenzymes A and B, and beta-galactosidase were determined by the method of Chatteriee et al in modification of Zwierz et al (mKat kg(-1) of protein). Protein was determined by the Lowry et al method (mg ml(-1)).

Results: The results presented here suggest that diabetes and smoking modify activity of HEX and its isoenzymes, but only combination of diabetes and smoking give a significant increase in the specific activity of HEX and its isoenzymes.

Conclusions: Type 1 diabetes slightly changes the composition of saliva. Smoking cigarettes significantly modifies the composition and properties of saliva in healthy individuals and patients with Type 1 diabetes.

MeSH terms

  • Adult
  • Analysis of Variance
  • Case-Control Studies
  • Diabetes Mellitus, Type 1 / enzymology*
  • Female
  • Hexosaminidase A
  • Hexosaminidase B
  • Humans
  • Isoenzymes
  • Lysosomes / enzymology
  • Male
  • Saliva / enzymology*
  • Salivary Proteins and Peptides / metabolism
  • Smoking / metabolism*
  • beta-Galactosidase / metabolism*
  • beta-N-Acetylhexosaminidases / metabolism*

Substances

  • Isoenzymes
  • Salivary Proteins and Peptides
  • beta-Galactosidase
  • Hexosaminidase A
  • Hexosaminidase B
  • beta-N-Acetylhexosaminidases