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Prostate. 2006 Sep 1;66(12):1268-74.

Generation of interleukin-17 receptor-like protein (IL-17RL) in prostate by alternative splicing of RNA.

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  • 1Center for Tissue Regeneration and Repair, Department of Orthopaedic Surgery, School of Medicine, University of California, Sacramento, California 95817, USA.

Abstract

BACKGROUND:

Interleukin-17 receptor-like protein (IL-17RL) expressed in prostate tissues changes with advanced cancers due to extensive alternative splicing, which affects the final protein. Predominant IL-17RL splice isoform variants have not been identified, hindering functional studies.

METHODS:

A cDNA library of IL-17RL transcripts was arrayed onto nylon membranes. Individual transcript exon structures were determined by successively probing membranes with exon-specific oligonucleotides. The most common variants were transiently over-expressed in 293T cells.

RESULTS:

We detected >90 different IL-17RL isoforms. Three most abundant isoforms account for approximately half the total transcripts; the full-length variant just over 11%. Surprisingly, most alternative splicing does not alter the reading frame of the full-length molecule; therefore, resulting proteins vary mostly in N-terminal domains.

CONCLUSIONS:

IL-17RL exists as multiple isoforms due to extensive alternative splicing. We identified the most abundant splices in prostate tissue and established a technique to investigate changes in RNA IL-17RL splicing that occur in advanced cancers.

(c) 2006 Wiley-Liss, Inc.

PMID:
16688746
[PubMed - indexed for MEDLINE]
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