Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt 5):457-9. Epub 2006 Apr 12.

Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering.

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Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.

Abstract

The large subunit of the essential pre-mRNA splicing factor U2 auxiliary factor (U2AF65) binds the polypyrimidine tract near the 3' splice site of pre-mRNA introns and directs the association of the U2 small nuclear ribonucleoprotein particle (U2 snRNP) of the spliceosome with the pre-mRNA. Protein engineering, in which the flexible linker region connecting tandem RNA-recognition motifs (RRMs) within the U2AF65 RNA-binding domain was partially deleted, allowed successful crystallization of the protein-nucleic acid complex. Cocrystals of a U2AF65 variant with a deoxyuridine dodecamer diffract X-rays to 2.9 angstroms resolution and contain one complex per asymmetric unit.

PMID:: 16682775; [PubMed - indexed for MEDLINE]
PMCID:: PMC2219975

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Cited by 6 PubMed Central articles

U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs. [Nucleic Acids Res. 2013]
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Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65. [J Biol Chem. 2008]
Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65.
Jenkins JL, Shen H, Green MR, Kielkopf CL. J Biol Chem. 2008 Nov 28; 283(48):33641-9. Epub 2008 Oct 8.

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Structures reported by this article

Structure Of U2af65 Variant With Polyuridine Tract

PDB: 2G4B

Source: Homo sapiens, synthetic construct

Method: X-Ray Diffraction

Resolution: 2.5 Å

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Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex wi...
Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1 ;62(Pt 5):457-9. Epub 2006 Apr 12 .

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Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering.

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