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Plant Physiol. 1991 Dec;97(4):1585-7.

Efficiency of particle-bombardment-mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco.

Author information

  • 1Sumitomo Chemical Co., Ltd., Biotechnology Laboratory, Takarazuka Research Center, 4-2-1 Takatsukasa, Takarazuka, Hyogo 665, Japan.

Abstract

Plasmid DNA pB1221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G(2) phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G(1) phases.

PMID:
16668589
[PubMed]
PMCID:
PMC1081205
Free PMC Article
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