Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Plant Physiol. 1990 Mar;92(3):637-41.

Subcellular compartmentation of uridine nucleotides and nucleoside-5' -diphosphate kinase in leaves.

Author information

  • 1Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, 8580 Bayreuth, Federal Republic of Germany.

Abstract

The subcellular compartmentation of nucleoside diphosphate kinase (EC 2.7.4.6) and the uridine nucleotides has been studied in leaves. Membrane filtration of barley (Hordeum vulgare L.) leaf mesophyll protoplasts and differential centrifugation of spinach (Spinacia oleracea L.) leaf extracts showed that about half the nucleoside diphosphate kinase is present in the cytosol. The activity is adequate to account for the turnover of UTP and UDP during photosynthetic sucrose synthesis. Nonaqueous density gradient centrifugation of freeze-stopped, lyophilized spinach leaf material showed that the uridine nucleotides are predominantly located in the cytosol and that the cytosolic UDP-glucose pool is considerably larger than the UTP or UDP pools.

PMID:
16667327
[PubMed]
PMCID:
PMC1062346
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk