Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Plant Physiol. 1990 Jan;92(1):155-9.

Characterization of ATP-Dependent Fructose 6-Phosphate 1-Phosphotransferase Isozymes from Leaf and Endosperm Tissues of Ricinus communis.

Author information

  • 1Department of Biology, Queen's University, Kingston, Ontario K7L 3N6 Canada.

Abstract

Plastid and cytosolic isozymes of ATP:fructose 6-phosphate 1-phosphotransferase (PFK(p) and PFK(c), respectively) have been isolated from leaves and developing endosperm tissues of the castor oil plant (Ricinus communis L). Endosperm PFK(p) has been purified to apparent homogeneity. Polyclonal antibodies raised against one of the four polypeptides associated with potato tuber PFK (molecular mass, 46 kilodaltons) immunoprecipitated developing endosperm and leaf PFK(p), but not PFK(c) isozymes. Western blots, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and analytical gel filtration show that PFK(p) from developing endosperm is a 220 kilodalton homotetramer composed of 57 kilodalton subunits. Kinetic studies of leaf PFK(p) and PFK(c) isozymes reveal both similarities and differences to the characteristics of the respective endosperm isozymes studied previously (WJ Garland, DT Dennis [1980] Arch Biochem Biophys 204: 302-317). The immunological and kinetic data suggest that leaf and developing endosperm PFK(p) are different but structurally related proteins.

PMID:
16667239
[PubMed]
PMCID:
PMC1062263
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk