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Carbohydr Res. 2006 Jul 24;341(10):1447-57. Epub 2006 May 2.

Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens.

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  • 1Glycan Array Synthesis Core D, Consortium for Functional Glycomics. The Scripps Research Institute, Department of Molecular Biology, CB 248A 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.


Poly-N-acetyllactosamines (pLNs) are common terminal sugars of many N- and O-linked glycan structures present in glycoproteins and glycolipids. Utilizing various glycosyltransferases, we developed new and efficient chemoenzymatic methods for the synthesis of pLNs in gram-scale. Specifically, the use of sialyltransferases and fucosyltransferases enabled us to synthesize and purify 24 blood group and tumor-associated pLN derivatives with alpha-(2-->3)- and alpha-(2-->6)-linked sialic acid, as well as with alpha-(1-->2)- and alpha-(1-->3)-linked fucose. All synthesized derivatives were linked to a short 2-azidoethyl spacer for further modification.

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