Abstract
Bipolar cells were recorded in rat retinal slices to study the distribution of hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels. Patch-clamp whole cell measurements were combined with intracellular filling and recorded cells were morphologically identified. HCN channel isoforms HCN1-4 are differentially expressed in bipolar cells. Each bipolar cell type has a characteristic inventory of HCN channels. The combination of HCN channel currents and other voltage-gated currents can be used as a kind of "finger print" to electrophysiologically identify and classify bipolar cell types. Using this approach of combined electrophysiological and morphological classification we could identify a new ON-cone bipolar cell type.
MeSH terms
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Animals
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Barium / pharmacology
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Cesium / pharmacology
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Cobalt / pharmacology
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Dose-Response Relationship, Radiation
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Electric Stimulation / methods
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Glycine / metabolism
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
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Immunohistochemistry / methods
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In Vitro Techniques
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Ion Channel Gating / physiology*
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Ion Channels / classification*
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Ion Channels / drug effects
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Ion Channels / metabolism
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Ion Channels / physiology*
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Ion Channels / radiation effects
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Membrane Potentials / radiation effects
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Patch-Clamp Techniques / methods
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Rats
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Retinal Bipolar Cells / classification*
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Retinal Bipolar Cells / metabolism
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Retinal Bipolar Cells / physiology*
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Retinal Bipolar Cells / radiation effects
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Vesicular Glutamate Transport Protein 1 / metabolism
Substances
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
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Ion Channels
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Vesicular Glutamate Transport Protein 1
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Cesium
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Barium
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Cobalt
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Glycine