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    Proteomics. 2006 May;6(10):2959-63.

    Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation.

    Sebela M, Stosová T, Havlis J, Wielsch N, Thomas H, Zdráhal Z, Shevchenko A.

    Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelů 11, 783 71 Olomouc, Czech Republic. sebela@prfholnt.upol.cz

    Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.

    PMID: 16637014 [PubMed - indexed for MEDLINE]

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