Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):520-6. Epub 2006 Apr 19.

Novel versatile cryoprotectants for heavy-atom derivatization of protein crystals.

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Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, Sayo-gun, Hyogo 679-5148, Japan.

Abstract

Determination of a suitable cryoprotectant agent condition is one of the time-consuming processes in protein X-ray crystallography. The adaptability of two novel versatile cryoprotectants (oil- and water-based types) has been evaluated by cryodiffraction experiments using crystals of five different proteins. The results suggest that complementary use of both oil- and water-based cryoprotectants enables the successful cryoprotection of protein crystals. Since the versatile cryoprotectants do not contain crystallization solution, rapid and efficient preparation of heavy-atom derivatives is possible with these cryoprotectants in the presence of heavy-atom reagents. Four heavy-atom reagents [K(2)PtCl(4), HgCl(2), KAu(CN)(2) and K(2)Pt(CN)(4)] were examined and could be stored with sufficient derivatizing capabilities for over one month in the versatile cryoprotectants.

PMID:: 16627945; [PubMed - indexed for MEDLINE]

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