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Biochim Biophys Acta. 2006 May;1764(5):856-62. Epub 2006 Apr 3.

NMR studies of BPTI aggregation by using paramagnetic relaxation reagents.

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  • 1Biomolecular Structure Research Center and Dipartimento di Biologia Molecolare, Universit√† di Siena, Via A. Fiorentina, I-53100 Siena, Italy.


Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovine pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates.

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