Format

Send to:

Choose Destination
See comment in PubMed Commons below
BMC Microbiol. 2006 Apr 18;6:35.

Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy.

Author information

  • 1Departamento de Biología Estructural, Instituto Venezolano de Investigaciones Científicas, Altos de Pipe, Estado Miranda, Venezuela. mcimino@ivic.ve

Abstract

BACKGROUND:

The establishment of the cellular localization of proteins in M. tuberculosis will provide of valuable information for the identification of new drug/vaccine/diagnostic targets. Cytolocalization by inmunofluorescence microscopy has been limited in mycobacteria because to difficulties in effectively permeabilize it.

RESULTS:

A treatment combining lysozyme with triton X-100 was found to be an effective permeabilization method of the mycobacterial envelope.

CONCLUSION:

A rapid and simple permeabilization protocol has been successfully assessed in pure cultures of both Mycobacterium smegmatis and Mycobacterium tuberculosis H37Rv. This method can be successful used in the cytolocalization of proteins by immunolabeling.

PMID:
16620389
[PubMed - indexed for MEDLINE]
PMCID:
PMC1458343
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Write to the Help Desk