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Biophys J. 2006 Jun 1;90(11):L80-2. Epub 2006 Apr 14.

Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells.

Author information

  • 1Chemical Biology Centre, Department of Physics, Imperial College London, London, United Kingdom. dylan.owen@imperial.ac.uk

Abstract

We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin.

PMID:
16617080
[PubMed - indexed for MEDLINE]
PMCID:
PMC1459501
Free PMC Article
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