An improved Tn7-based system for the single-copy insertion of cloned genes into chromosomes of gram-negative bacteria

Y Bao; D P Lies; H Fu; G P Roberts

doi:10.1016/0378-1119(91)90604-a

An improved Tn7-based system for the single-copy insertion of cloned genes into chromosomes of gram-negative bacteria

Gene. 1991 Dec 20;109(1):167-8. doi: 10.1016/0378-1119(91)90604-a.

Authors

Y Bao¹, D P Lies, H Fu, G P Roberts

Affiliation

¹ Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.

PMID: 1661697
DOI: 10.1016/0378-1119(91)90604-a

Abstract

A system is described for the single-copy, stable insertion of cloned DNA sequences into the chromosomes of Gram- bacteria. Two narrow-host-range plasmids form the basis of this system: the 'carrier' plasmid contains the mini Tn7-Km transposon, into which foreign DNA can be cloned; the 'helper' plasmid provides the Tn7 transposition functions in trans. Both plasmids are readily transferred into Gram- bacteria by conjugation. The functionality of this system has been demonstrated in Rhodospirillum rubrum.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Chromosomes, Bacterial*
Cloning, Molecular*
DNA Transposable Elements / genetics*
Gram-Negative Bacteria / genetics*
Plasmids / genetics
Rhodospirillum rubrum / genetics

Substances

DNA Transposable Elements