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Rev Med Chir Soc Med Nat Iasi. 2005 Jul-Sep;109(3):664-7.

[Quantification of caffeine from energy drinks with mass spectrometry method (LC/MS)].

[Article in Romanian]

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  • 1Universitatea de Medicină şi Farmacie Iuliu Haţieganu Cluj-Napoca, Facultatea de Farmacie, Disciplina de Chimie Sanitară-Igienă.


A high performance liquid chromatography coupled with mass spectrometry method (LC/MS) for quantification of caffeine from energy drinks was elaborated. It was utilised a Zorbax SB-C18, 100 mm x 3.0 mm i.d., 3.5 microm column with a mobile phase containing methanol/ solution 0.2% formic acid in water. Detection and quantification of caffeine was based on monitoring the protonated molecular ion abundance (m/z 194.9). The quantification was made using the external standard method. The calibration curve were made on range 0.26-26 microg/ml for caffeine with a correlation coefficient greater than 0.995. There were analysed 12 energy drinks with a caffeine content between 13 and 34 mg/100 ml.

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