Abstract
The high-throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self-assembly concepts. DNA-directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O6-alkylguanine-DNA-alkyltransferase (SNAP-tag) and the Avitag. Use of the conjugates in converting a DNA chip into a protein chip by self assembly is demonstrated.
MeSH terms
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Base Sequence
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Escherichia coli / genetics
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Feasibility Studies
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Molecular Probes / chemistry*
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Molecular Sequence Data
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Molecular Structure
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O(6)-Methylguanine-DNA Methyltransferase / chemistry
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Oligonucleotide Array Sequence Analysis / instrumentation
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Oligonucleotide Array Sequence Analysis / methods*
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Protein Array Analysis / instrumentation
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Protein Array Analysis / methods*
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Qb-SNARE Proteins / chemistry
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Qc-SNARE Proteins / chemistry
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Recombinant Fusion Proteins / chemistry*
Substances
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Molecular Probes
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Qb-SNARE Proteins
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Qc-SNARE Proteins
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Recombinant Fusion Proteins
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SNAP23 protein, human
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O(6)-Methylguanine-DNA Methyltransferase