Lasers Surg Med. 2006 Apr;38(4):305-13.

Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model.

Hariri LP, Tumlinson AR, Besselsen DG, Utzinger U, Gerner EW, Barton JK.

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Division of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA.

Abstract

BACKGROUND AND OBJECTIVES:

The diagnostic feasibility of optical coherence tomography (OCT) and laser-induced fluorescence (LIF) have been evaluated for human colorectal cancer. This study applies these technologies to a murine model of colorectal adenoma.

STUDY DESIGN/MATERIALS AND METHODS:

The lower colon of 10 Apc(Min) and two C57BL/6J mice was surveyed over five 4-week intervals using a prototype 2.0 mm diameter OCT-LIF endoscope-based system. Four categories were histologically classified: control C57BL/6J, adenomatous, non-diseased regions of adenomatous, and non-diseased Apc(Min). OCT images were compared to histology. Spectra from the four categories were compared via the Student's t-test.

RESULTS:

Three Apc(Min) and two control mice completed the study. One adenoma was histologically identified; OCT visualized mucosal thickening/abnormal mass development over the imaging timepoints. LIF spectral comparisons revealed decreased 405 nm intensity and the presence of a peak at 680 nm in the adenomatous Apc(Min).

CONCLUSIONS:

These preliminary data indicate endoscopic OCT-LIF has the potential to identify colorectal adenomas in murine models.

PMID:: 16596657; [PubMed - indexed for MEDLINE]

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