Sequence alignment of M. jannaschii lactaldehyde dehydrogenase with lactaldehyde dehydrogenases from E. coli (encoded by aldB, NP_418045) and Salmonella enterica serovar Typhimurium (encoded by aldB, NP_462580) as well as T. tenax glyceraldehyde-3-phosphate dehydrogenase (T44939). Identical residues are shown in boldface, and similar residues are highlighted in gray. Conserved catalytic cysteine and glutamate residues are indicated with an asterisk. Residues proposed to be involved in glyceraldehyde-3-phosphate dehydrogenase substrate binding are highlighted in black.

Fuculose-1-phosphate aldolase reaction products. Shown is an analysis of the reduced and dephosphorylated reaction products resulting from the condensation of d- and l-lactaldehyde with DHAP. The four peaks observed were generated by reduction of the two products resulting from the condensation of DHAP with l- and d-lactaldehyde, respectively. (A) GC-MS fragmentation pattern of unlabeled TMS₅ products. (B) GC-MS fragmentation pattern of products reduced with borodeuteride. (C) GC-MS fragmentation pattern of product formed from lactaldehyde labeled with deuterium on C-2. Although the fragmentation pattern is drawn for the fucitol (TMS)₅ derivative, an identical fragmentation pattern was seen in the corresponding peak for each stereoisomer.

Pathway for lactate formation in M. jannaschii.