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Anal Chem. 2006 Apr 1;78(7):2272-8.

Quantitative 3D mapping of fluidic temperatures within microchannel networks using fluorescence lifetime imaging.

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  • 1Department of Physics and Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, United Kingdom.

Abstract

We describe a novel method for quantitatively mapping fluidic temperature with high spatial resolution within microchannels using fluorescence lifetime imaging in an optically sectioning microscope. Unlike intensity-based measurements, this approach is independent of experimental parameters, such as dye concentration and excitation/detection efficiency, thereby facilitating quantitative temperature mapping. Micrometer spatial resolution of 3D temperature distributions is readily achieved with an optical sectioning approach based on two-photon excitation. We demonstrate this technique for mapping of temperature variations across a microfluidic chip under different heating profiles and for mapping of the 3D temperature distribution across a single microchannel under applied flow conditions. This technique allows optimization of the chip design for miniaturized processes, such as on-chip PCR, for which precise temperature control is important.

PMID:
16579608
[PubMed - indexed for MEDLINE]
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