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J Am Chem Soc. 2006 Apr 5;128(13):4206-7.

Metal-enhanced fluorescence-based RNA sensing.

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  • 1Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, University of Maryland School of Medicine, Baltimore, 21201, USA.

Abstract

In this communication, we present the proof-of-principle of a new RNA sensing technique, based on metal-enhanced fluorescence, which may improve the potential of solid substrate-based RNA capture assays. In this regard, the detection of RNA is accomplished by annealing a target RNA tagged with a fluorophore to a DNA anchor probe in a single step on a solid surface, where the fluorescence signal is enhanced by silver nanoparticles. Furthermore, the proposed method is highly amenable to high-throughput screening applications and is capable of detecting less than 25 fmol of RNA at a signal-to-noise ratio greater than 20, in several hours compared to that of reverse transcription-PCR at >24 h.

PMID:
16568977
[PubMed - indexed for MEDLINE]
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