Objective: To study the optimal extraction process of polysaccharide from Baphicacanthus cuaia (Nees) Bremek.
Methods: The optimal extraction process was selected with uniform design and statistical analysis system (SAS). The content of polysaccharide in the extract was determined to investigate the influence of temperature of extraction, the time of extraction and the solid-liquid ratio on it.
Results: The optimal extraction process was extraction temperature 90 degrees C with 2 hours and with 1:50 (g/ml) of Baphicacanthus cuaia (Nees) Bremek weight to distilled water. The optimum extraction rates of Polysaccharide from Baphicacanthus cuaia (Nees) Bremek and Isatis indigotica Fort were 3.26% and 12.76%.