J Biochem. 2006 Mar;139(3):323-7.

Application of lectin microarray to crude samples: differential glycan profiling of lec mutants.

Ebe Y, Kuno A, Uchiyama N, Koseki-Kuno S, Yamada M, Sato T, Narimatsu H, Hirabayashi J.

Source

Nano/Bio Science Research Lab., Yokohama Technical Center, Moritex Corporation, 1-3-3 Azamino-Minami, Aoba-ku, Yokohama, Kanagawa 225-0012.

Abstract

We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.

PMID:: 16567396; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

A novel strategy for mammalian cell surface glycome profiling using lectin microarray. [Glycobiology. 2007]
A novel strategy for mammalian cell surface glycome profiling using lectin microarray.
Tateno H, Uchiyama N, Kuno A, Togayachi A, Sato T, Narimatsu H, Hirabayashi J. Glycobiology. 2007 Oct; 17(10):1138-46. Epub 2007 Aug 10.
Focused differential glycan analysis with the platform antibody-assisted lectin profiling for glycan-related biomarker verification. [Mol Cell Proteomics. 2009]
Focused differential glycan analysis with the platform antibody-assisted lectin profiling for glycan-related biomarker verification.
Kuno A, Kato Y, Matsuda A, Kaneko MK, Ito H, Amano K, Chiba Y, Narimatsu H, Hirabayashi J. Mol Cell Proteomics. 2009 Jan; 8(1):99-108. Epub 2008 Aug 11.
A versatile technology for cellular glycomics using lectin microarray. [Methods Enzymol. 2010]
A versatile technology for cellular glycomics using lectin microarray.
Tateno H, Kuno A, Itakura Y, Hirabayashi J. Methods Enzymol. 2010; 478:181-95.
Review Lectin-resistant CHO glycosylation mutants. [Methods Enzymol. 2006]
Review Lectin-resistant CHO glycosylation mutants.
Patnaik SK, Stanley P. Methods Enzymol. 2006; 416:159-82.
Review Concept, strategy and realization of lectin-based glycan profiling. [J Biochem. 2008]
Review Concept, strategy and realization of lectin-based glycan profiling.
Hirabayashi J. J Biochem. 2008 Aug; 144(2):139-47. Epub 2008 Apr 4.

See reviews... See all...

Cited by 5 PubMed Central articles

High-throughput lectin microarray-based analysis of live cell surface glycosylation. [Curr Protoc Protein Sci. 2011]
High-throughput lectin microarray-based analysis of live cell surface glycosylation.
Li Y, Tao SC, Zhu H, Schneck JP. Curr Protoc Protein Sci. 2011 Feb; Chapter 12:Unit12.9.
Lectin microarray. [Proteomics Clin Appl. 2009]
Lectin microarray.
Hu S, Wong DT. Proteomics Clin Appl. 2009 Feb; 3(2):148-154.
Review Carbohydrate recognition by boronolectins, small molecules, and lectins. [Med Res Rev. 2010]
Review Carbohydrate recognition by boronolectins, small molecules, and lectins.
Jin S, Cheng Y, Reid S, Li M, Wang B. Med Res Rev. 2010 Mar; 30(2):171-257.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Application of lectin microarray to crude samples: differential glycan profiling...
Application of lectin microarray to crude samples: differential glycan profiling of lec mutants.
J Biochem. 2006 Mar ;139(3):323-7.

PubMed
Clostridium septicum infections in children: a case report and review of the lit...
Clostridium septicum infections in children: a case report and review of the literature.
Pediatrics. 2006 Apr ;117(4):e796-805. Epub 2006 Mar 27 .

PubMed
Synovial fibroblasts: key players in rheumatoid arthritis.
Synovial fibroblasts: key players in rheumatoid arthritis.
Rheumatology (Oxford). 2006 Jun ;45(6):669-75. Epub 2006 Mar 27 .

PubMed
False-positive rate in prenatal diagnosis of surgical anomalies.
False-positive rate in prenatal diagnosis of surgical anomalies.
J Pediatr Surg. 2006 Apr ;41(4):826-9.

PubMed
Obesity, disordered eating, and eating disorders in a longitudinal study of adol...
Obesity, disordered eating, and eating disorders in a longitudinal study of adolescents: how do dieters fare 5 years later?
J Am Diet Assoc. 2006 Apr ;106(4):559-68.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: