Interaction of lambda Gam protein with the RecD subunit of RecBCD enzyme increases radioresistance of the wild-type Escherichia coli

Biochimie. 1991 Apr;73(4):501-3. doi: 10.1016/0300-9084(91)90119-l.

Abstract

By making use of the gam(+)-plasmid, the so-called gam-dependent radioresistance was studied. This resistance is the result of the interaction between Gam protein (encoded by the gam gene of lambda) and RecBCD enzyme of Escherichia coli. gam-dependent radioresistance is observed in recB+ recC+ recD+ but not in recB+ recC+ recD- cells. It is suggested that Gam protein interacts specifically with the RecD subunit of RecBCD enzyme; the RecBC complex probably retains its activity in the presence of this viral protein.

MeSH terms

  • Bacteriophage lambda / genetics*
  • Bacteriophage lambda / radiation effects
  • DNA Helicases / genetics*
  • DNA Helicases / radiation effects
  • DNA-Binding Proteins
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / radiation effects*
  • Escherichia coli Proteins*
  • Exodeoxyribonuclease V
  • Exodeoxyribonucleases / genetics*
  • Exodeoxyribonucleases / radiation effects
  • Gamma Rays
  • Plasmids
  • Viral Proteins / genetics*
  • Viral Proteins / radiation effects

Substances

  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Viral Proteins
  • gam protein, Coliphage
  • Exodeoxyribonucleases
  • Exodeoxyribonuclease V
  • exodeoxyribonuclease V, E coli
  • DNA Helicases