RNA. 2006 May;12(5):913-20. Epub 2006 Mar 15.

A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA).

Castoldi M, Schmidt S, Benes V, Noerholm M, Kulozik AE, Hentze MW, Muckenthaler MU.

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Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany.

Abstract

MicroRNAs represent a class of short (approximately 22 nt), noncoding regulatory RNAs involved in development, differentiation, and metabolism. We describe a novel microarray platform for genome-wide profiling of mature miRNAs (miChip) using locked nucleic acid (LNA)-modified capture probes. The biophysical properties of LNA were exploited to design probe sets for uniform, high-affinity hybridizations yielding highly accurate signals able to discriminate between single nucleotide differences and, hence, between closely related miRNA family members. The superior detection sensitivity eliminates the need for RNA size selection and/or amplification. MiChip will greatly simplify miRNA expression profiling of biological and clinical samples.

PMID:: 16540696; [PubMed - indexed for MEDLINE]
PMCID: PMC1440900

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miChip: a microarray platform for expression profiling of microRNAs based on locked nucleic acid (LNA) oligonucleotide capture probes. [Methods. 2007]
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Castoldi M, Benes V, Hentze MW, Muckenthaler MU. Methods. 2007 Oct; 43(2):146-52.
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A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA).

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