Biotechnol Bioeng. 2006 May 5;94(1):1-4.

A tightly regulated inducible expression system utilizing the fim inversion recombination switch.

Source

Department of Bioengineering, University of California, Berkeley, 94720, USA.

Abstract

The fim inversion system of Escherichia coli (E. coli) can behave as a unidirectional switch in an efficient manner. We have developed a new expression system for E. coli, comprising the arabinose-inducible fimE gene and the fim invertible DNA segment containing a constitutively active promoter. In this system, the target gene is cloned with the promoter in the OFF orientation, resulting in no transcribed product. When induced by arabinose, the active promoter is switched to the ON orientation via FimE-catalyzed DNA inversion, and the gene is expressed. Our expression system exhibited very tightly controlled basal expression and high induced expression, with simple induction by inexpensive arabinose. These characteristics make our system suitable for large-scale expression or for production of toxic proteins.

PMID:: 16534780; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Fimbriae Proteins

Grant Support

5R01GM063525-03/GM/NIGMS NIH HHS/United States

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Addgene: plasmid: pFIP insert: inversion switch - Neuroscience Information Framework

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Orientational control of fimE expression in Escherichia coli. [Mol Microbiol. 2001]
Orientational control of fimE expression in Escherichia coli.
Sohanpal BK, Kulasekara HD, Bonnen A, Blomfield IC. Mol Microbiol. 2001 Oct; 42(2):483-94.
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Inversion-independent phase variation of type 1 fimbriae in Escherichia coli. [J Bacteriol. 1993]
Inversion-independent phase variation of type 1 fimbriae in Escherichia coli.
McClain MS, Blomfield IC, Eberhardt KJ, Eisenstein BI. J Bacteriol. 1993 Jul; 175(14):4335-44.
Review The regulation of pap and type 1 fimbriation in Escherichia coli. [Adv Microb Physiol. 2001]
Review The regulation of pap and type 1 fimbriation in Escherichia coli.
Blomfield IC. Adv Microb Physiol. 2001; 45:1-49.
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