Level of M(IP)2C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast

FEBS Lett. 2006 Mar 20;580(7):1903-7. doi: 10.1016/j.febslet.2006.02.061. Epub 2006 Mar 3.

Abstract

The antifungal plant defensin DmAMP1 interacts with fungal sphingolipids of mannosyldiinositolphosphorylceramide (M(IP)2C) class. We screened a Saccharomyces cerevisiae transposon (Tn) mutant library against DmAMP1 and identified one DmAMP1-resistant mutant with the Tn inserted in the M(IP)2C biosynthesis gene IPT1 (DmTn11) and one DmAMP1-hypersensitive mutant with the Tn inserted in rDNA (HsTnII). However, tetrad analysis pointed to HsTnII as a spontaneous mutant. Apparently, membranes of DmTn11 lack M(IP)2C, whereas membranes of HsTnII have increased M(IP)2C levels. In addition, DmTn11 and HsTnII are characterized by increased and reduced oxidative stress resistance/chronological life-span (CL), respectively. A putative involvement of M(IP)2C in oxidative stress and CL in yeast is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Defensins / physiology*
  • Glycosphingolipids / analysis
  • Glycosphingolipids / physiology*
  • Mutation
  • Oxidative Stress*
  • Oxygen / metabolism*
  • Plants / immunology
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics
  • Yeasts / cytology*

Substances

  • Defensins
  • Glycosphingolipids
  • mannose-bis(inositolphospho)ceramide
  • Oxygen