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    EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9.

    Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY.

    Mirza O, Guan L, Verner G, Iwata S, Kaback HR.

    Source

    Department of Biological Sciences, Membrane Protein Crystallography Group, Imperial College London, London, UK.

    Erratum in

    • EMBO J. 2006 May 3;25(9):2038.

    Abstract

    Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.

    PMID:
    16525509
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC1422171
    Free PMC Article

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