Global gene co-expression networks. (A-C) Log-log plots of connectivity distributions in each of the three networks drawn from DNA Damage, Environmental Response, and Cell Cycle, respectively. The linear relationship indicates a scale-free structure. (D-F) Correlation plots showing the relationship between gene group connectivity and essentiality in the same order as A-C. On the y axis of these plots is the percentage of genes determined to be essential in yeast. (G-I) Relationship between the average blastp score of a node and k for all datasets in the same order as A-C. On the y axis of these plots is the average log(e score) of genes within that bin. For plots D-I, the connectivity within each network for each gene was determined and each gene rank ordered by connectivity. 20 equal sized bins for each of the expression datasets were formed and the average connectivity of the genes in each bin plotted on the X axis

A co-expression network of the Environmental Response dataset. For all panels in the figure: blue color represents members of the rRNA processing module, yellow color represents members of the protein synthesis module and red color represents members of the ubiquitin pathway. (A) A hierarchical clustering of the topological overlap matrix for the ER dataset. (B) A drawn network of gene co-expression from the ER dataset. A drawn network of gene co-expression from the DD dataset. Edges were computed from the Pearson correlation coefficients. Network structure was drawn in Pajek [20]. Each gene is represented as a dot. Edges are drawn as grey lines. Colored dots belong to the module that their color indicates and grey dots indicate all other genes in the network. (C-E) Correlation plots showing the relationship between gene group connectivity and essentiality for rRNA processing, protein synthesis and ubiquitin from the ER network respectively. On the y axis of these plots is the percentage of genes determined to be essential in yeast. The number of essential genes in each module/total number of genes in each module was: 317/929 = 34.1 (C), 98/323 = 30.3 (D), 18/37 = 48.6 (E). (F-H) Relationship between the average blastp score of a node and k for the same module members listed in C-E. On the y axis of these plots is the average log(e score) of genes within that bin. For plots C-H, the connectivity within each module for each gene was determined and each gene rank ordered by connectivity.

Module members retrieved from very different datasets are conserved. For all panels in the figure: black color represents modules retrieved from the DD dataset, blue color represents modules retrieved from the ER dataset, and green color represents modules retrieved from the CC dataset. In each panel A-C a different module is examined for overlap of its members in a Venn diagram. In each diagram the relative overlap of the various modules has been diagramed as different amounts of area with the count represented for each zone by a number that quantifies the precise number of matches.

Generating a gene co-expression network. (A) Illustration to show how genes that are highly correlated (blue) will look across a dataset. (B-C) Flow chart for defining a gene co-expression network based on a simple Pearson correlation matrix. (D) An example visualization of a network produced by using the Fruchterman Rheingold algorithm on the data in Figure 1B and 1C. The node highlighted in yellow, is an example of the kind of highly connected node that this study shows is more likely to be an essential gene for survival.

A co-expression network of the DNA Damage dataset. For all panels in the figure: blue color represents members of the rRNA processing module, yellow color represents members of the protein synthesis module and red color represents members of the ubiquitin pathway. (A) A hierarchical clustering of the topological overlap matrix for the DD dataset. (B) A drawn network of gene co-expression from the DD dataset. Edges were computed from the Pearson correlation coefficients. Network structure was drawn in Pajek [20]. Each gene is represented as a dot. Edges are drawn as grey lines. Colored dots belong to the module that their color indicates and grey dots indicate all other genes in the network. (C-E) Scatter plots showing the relationship between gene group connectivity and essentiality for rRNA processing, protein synthesis and ubiquitin from the DD network respectively. On the y axis of these plots is the percentage of genes determined to be essential in yeast. The number of essential genes in each module/total number of genes in each module was: 196/390 = 50.3 (C), 122/441 = 27.7 (D), 50/222 = 22.5 (E). (F-H) Relationship between the average blastp score of a node and k for the same module members listed in C-E. On the y axis of these plots is the average log(e score) of genes within that bin. For plots C-H, the connectivity within each module for each gene was determined and each gene rank ordered by connectivity. 20 equal sized bins for each of the expression datasets were formed and the average connectivity of the genes in each bin plotted on the X axis.

A co-expression network of the Cell Cycle dataset. For all panels in the figure: blue color represents members of the rRNA processing module, yellow color represents members of the protein synthesis module and red color represents members of the ubiquitin pathway. (A) A hierarchical clustering of the topological overlap matrix for the CC dataset. (B) A drawn network of gene co-expression from the CC dataset. A drawn network of gene co-expression from the DD dataset. Edges were computed from the Pearson correlation coefficients. Network structure was drawn in Pajek [20]. Each gene is represented as a dot. Edges are drawn as grey lines. Colored dots belong to the module that their color indicates and grey dots indicate all other genes in the network. (C-E) Correlation plots showing the relationship between gene group connectivity and essentiality for rRNA processing, protein synthesis and ubiquitin from the CC network respectively. On the y axis of these plots is the percentage of genes determined to be essential in yeast. The number of essential genes in each module/total number of genes in each module was: 154/300 = 51.3 (C), 105/398 = 26.4 (D), 38/312 = 12.2 (E). (F-H) Relationship between the average blastp score of a node and k for the same module members listed in C-E. On the y axis of these plots is the average log(e score) of genes within that bin. For plots C-H, the connectivity within each module for each gene was determined and each gene rank ordered by connectivity.