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Clin Chem. 2006 Mar;52(3):520-3.

Identification of mRNA markers for molecular staging of lymph nodes in colorectal cancer.

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  • 1Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Abstract

BACKGROUND:

One evolving approach to improved prognostication of cancer patients is the identification of previously occult disease by use of quantitative reverse transcription-PCR. Surprisingly, no systematic analysis of potential mRNA markers for colorectal cancer has been reported. We therefore performed an extensive mRNA marker survey for colorectal cancers.

METHODS:

We identified potential markers through literature and database searches. We analyzed all markers by quantitative reverse transcription-PCR on a limited set of primary tumors and benign lymph nodes. Selected markers were further evaluated on a larger tissue set with positive lymph nodes.

RESULTS:

We evaluated 43 markers and undertook further analysis of 6 in the secondary screening. Five gene markers--CDX1, carcinoembryonic antigen (CEA), CK20, TACSTD1, and Villin1 (VIL1)--provided perfect classification of lymph node status.

CONCLUSIONS:

Several mRNA markers are capable of providing exceptionally accurate characterization of lymph node status in colorectal cancer. An automated, multimarker, quantitative reverse transcription-PCR assay for characterization of lymph nodes from colorectal cancer patients may be useful for improved staging and therapeutic decision making in colorectal cancer.

PMID:
16510433
[PubMed - indexed for MEDLINE]
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